adaA [2019-08-27 11:53:59]

trigger enzyme

Locus: BSU_01810

Isoelectric point: 9.19

Molecular weight: 24.15 kDa

Protein length: 211 aa

Gene length: 636 bp

Function: adaptive response to alkylative DNA damage

Product: trigger enzyme

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes that act directly as transcription factors by binding DNA
• Prophages and mobile genetic elements → Prophages → Prophage 1

Gene

Coordinates: 203,729 204,364

The protein

Catalyzed reaction/ biological activity

• methylphosphotriester deoxyribonucleoside in DNA + L-cysteinyl-[protein] --> deoxyribonucleotide in DNA + H⁺ + S-methyl-L-cysteinyl-[protein] (according to UniProt)

Protein family

• AraC family

Domains

• HTH araC/xylS-type domain (aa 102-200) (according to UniProt)

Structure

• 1ZGW (from E. coli, 39% identity) PubMed

Expression and Regulation

Operons

• Genes: adaA-adaB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AdaA: positive regulation, in AdaA regulon

  Regulation

  • positive control by AdaA PubMed

Biological materials

Mutant

• BKE01810 (adaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC
• BKK01810 (adaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC

References

Reviews

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Original publications

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